Coding

Part:BBa_K2398000:Design

Designed by: Lukas Platz   Group: iGEM17_Heidelberg   (2017-10-25)


Organosilicon catalyzing Cytochrome c


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had to be compatible with the Biobrick standard. Therefore, Biobrick prefix and suffix was added upstream and downstream of the coding sequence.


Source

The Cytochrome c is derived from the Gram-negative bacteria Rhodotermus marinus and is codon-optimized for the use in Escherichia coli. It was ordered as gBlock from IDT (Integrated DNA Technologies).

References